About

Using molecular biology to solve complex biological problems

The Stachura Laboratory started in 2014 when Dr. David Stachura moved to Chico, CA and became an Assistant Professor at Chico State. He quickly earned tenure and advanced to Associate Professor, and soon after to Full Professor. Dr. Stachura earned a multitude of awards at Chico State and ran one of the most successful laboratories on campus. In fact, more graduate students have gotten their degrees in the Stachura Lab in 10 years than most other professors mentor in the whole career.

While Chico State is a primarily teaching institution, Dr. Stachura's teaching pedagogy led him to integrate his research into the classroom every chance that he could get. That resulted in him developing new classes focused on research and hands-on, impactful science for students to participate in. This focus on performing science generated many publications featuring students, many local, national, and international presentations featuring students, and even integrating real experiments into the local middle schools here in Chico. Dr. Stachura also consults with local biotechnology companies and introduced students to this side of science. Want to know more about the most successful laboratory on campus? Read on!

Publications from the Stachura Laboratory

While David Stachura has a multitude of publications, many of those publications came from his time at Chico State. Importantly, they involved undergraduate and Master's students in his lab (and others across the campus).

In the publications below, all undergraduates in the Stachura Laboratory are underlined and all Master's students in the Stachura Laboratory are italicized. Shared authorship is denoted by "*".

Journal Articles

Campbell C, Su T, Lau RP, Shah A, Laurie PC, Avalos B, Aggio J, Harris E, Traver D, Stachura DL. 2015.
Zebrafish embryonic stromal trunk (ZEST) cells support hematopoietic stem and progenitor cell (HSPC) proliferation, survival, and proliferation. Experimental Hematology. 2015 Dec;43(12):1047-61.

Harris EY, Keller D, Stachura D, Wolfe G. 2016. Bioinformatics sister courses: an interdisciplinary
collaborative learning framework to teach bioinformatics. Computer Science and Computational
Intelligence. 2016.

Svoboda O, Stachura DL, Machonova O, Zon LI, Traver D, Bartunek P. 2016. Ex vivo tools for the clonal analysis of zebrafish hematopoiesis. Nature Protocols. 2016 Apr 28;11:1007-20.

Kim JH, Shinde DN, Reijnders MRF, Hauser NS, Belmonte RL, Wilson GR, Bosch DGM, Bubulya PA, Shashi V, Petrovski S, Stone JK, Park EY, Veltman JA, Sinnema M, Stumpel C, Draaisma JM, Nicolai J, Yntema HG, Lindstrom K, de Vries BBA, Jewett T, Santoro SL, Vogt J, Bachman KK, Seeley AH, Krokosky A, Turner C, Rohena L, Hempel M, Kortüm F, Lessel D, Neu A, Strom TM, Wieczorek D, Bramswig N, Laccone FA, Behunova J, Rehder H, Gordon CT, Rio M, Romana S, Tang S, El-Khechen D, Cho MT, McWalter K, Douglas G, Baskin B, Begtrup A, Funari T, Schoch K, Stegmann APA, Stevens SJC, Zhang DE, Traver D, Yao X, MacArthur DG, Brunner HG, Mancini GM, Myers RM, Owen LB, Lim ST, Stachura DL, Vissers L, Ahn EY. 2016. De novo loss-of-function mutations in SON disrupt RNA-splicing of genes essential for brain development and metabolism, causing an intellectual disability syndrome. The American Journal of Human Genetics. 2016 Sep 1;99(3):711-9.

Cortes M, Chen MJ, Stachura DL, Liu SY, Kwan W, Wright F, Vo LT, Theodore LN, Esain V, Frost IM,
Schlaeger TM, Goessling W, Daley GQ, North TE. 2016. Developmental vitamin D availability impacts
hematopoietic stem cell production. Cell Rep. 2016 Oct 4;17(2):458-468.

Gansner JM, Leung AD, Superdock M, Blair MC, Ammerman MB, Durand EM, Barut B, Handin RI, Stachura DL, Lu C, Springer TA, Zon LI. 2017. Sorting zebrafish thrombocyte lineage cells with a cd41 monoclonal antibody enriches hematopoietic stem cell activity. Blood. 2017 Mar 9;129(10):1394-1397.

Wolf A*, Aggio J*, Campbell C, Wright F, Marquez G, Traver D, Stachura DL. 2017. Zebrafish caudal
haematopoietic embryonic stromal tissue (CHEST) cells support haematopoiesis. Sci Rep. 2017 Mar
16;7:44644.

Kasheta M, Painter CA, Moore FE, Lobbardi R, Bryll A, Freiman E, Stachura D, Rogers AB, Houvras Y,
Langenau DM, Ceol CJ. 2017. Identification and characterization of T reg-like cells in zebrafish. J Exp
Med. 2017 Dec 4;214(12):3519-3530.

Berrun AC, Stachura DL. 2017. Development of an in vitro assay to quantitate hematopoietic stem and progenitor cells (HSPCs) in developing zebrafish embryos. J Vis Exp. 2017 Nov 30;(129).

Liu C, Han T, Stachura DL, Wang H, Vaisman BL, Kim J, Klemke RL, Remaley AT, Rana TM, Traver D,
Miller YI. 2018. Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply. Nat Commun. 2018 Apr 3;9(1):1310.

Berrun AC, Harris E, Stachura DL. 2018. isthmin 1 (ism1) is required for normal hematopoiesis in
developing zebrafish. PLoS One. 2018 May 14;13(5):e0196872.

Rost MS, Shestopalov I, Liu Y, Vo AH, Richter CE, Emly SM, Barrett FG, Stachura DL, Holinstat M, Zon
LI, Shavit JA. 2018. Nfe2 is dispensable for early but required for adult thrombocyte formation and
function in zebrafish. Blood Adv. 2018 Dec 11;2(23):3418-3427.

Kim JH, Park EY, Chitayat D, Stachura DL, Schaper J, Lindstrom K, Jewett T, Wieczorek D, Draaisma JM, Sinnema M, Hoeberigs C, Hempel M, Bachman KK, Seeley AH, Stone JK, Kong HK, Vukadin L, Richard A, Shinde DN, McWalter K, Si YC, Douglas G, Lim ST, Vissers LELM, Lemaire M, Ahn EE. 2019. SON haploinsufficiency causes impaired pre-mRNA splicing of CAKUT genes and heterogeneous renal phenotypes. Kidney Int. 2019 Mar 15.

Cajias I*, Belmonte B*, Maciel S*, Thapa S*, Tomasetti S, Aggio J, Stachura DL. 2019. Different wavelengths of light have no effect on zebrafish fecundity. Science Matters. 2019 Apr 18. 10.19185/matters.201904000001

Lewis TR, Smith J, Griffin K, Aguiar S, Rueb KF, Holmberg-Douglas N, Sampson EM, Tomasetti S, Rodriguez S, Stachura DL*, Arpin CC*. 2020. NHD2-15, a novel antagonist of Growth Factor Receptor-Bound Protein-2 (GRB2), inhibits leukemic proliferation. PLoS One. 2020 Aug 11.

Belmonte RL, Engbretson I, Kim JH, Cajias I, Ahn EY, Stachura DL. 2021. son is necessary for proper
vertebrate blood development. PLoS One. 2021 Feb 25;16(2):e0247489.

Rueb KF, Stachura DL. 2021. Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish. J Vis Exp. 2021 Apr 29.

Thapa S, Stachura DL. 2021. Deep learning approach for quantification of fluorescently labeled blood cells in Danio rerio (zebrafish). Bioinformatics and Biology Insights. 2021 Jul 15; 15: 1-9.

Namit A*, Dowell W*, Matiasek S, Webster J, Stachura DL. 2022. Pentachlorophenol has significant adverse effects on hematopoietic and immune system development in zebrafish (Danio rerio). PLoS One. 2022 Mar 25;17(3):e0265618.

Review Articles

Griffin K, Cajias I, Aguiar S, Tomasetti S, Stachura DL. 2019. How can you tell that stem cells are good
at math? You can watch them multiply and divide! Atlas of Science. 2019 Jan 15.

Rubio N, Datar I, Stachura D, Kaplan D, Krueger K. 2019. Cell-based fish: A novel approach to seafood production and an opportunity for cellular agriculture. Frontiers in Sustainable Food Systems, 11 June 2019.

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Publications from the Stachura Laboratory

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